Table 2. The list of various media tested in this experiment

Type of strain Type of media (or agar) Characteristic
Bacillus cereus Mannitol-Yolk-Polymyxin B (MYP) Which contains egg yolk and polymyxin B for selective growth and differentiation.
Polymyxin Egg Yolk Mannitol Bromothymol Blue (PEMBA) Which provides enhanced visual distinction of B. cereus colonies through pH changes and lecithinase activity.
Commercial chromogenic media Which allow for more specific identification through color-based enzyme activity detection.
Clostridium perfringens Tryptose Sulfite Cycloserine (TSC) Which enables the detection of sulfite-reducing colonies under anaerobic conditions.
Shahidi-Ferguson Perfringens (SFP) Another sulfite-reducing medium that contains egg yolk for lecithinase differentiation.
Commercial chromogenic media Which provide improved specificity by targeting C. perfringens-specific enzyme activity.
Staphylococcus aureus Mannitol Salt Agar (MSA) Which selects for Staphylococcus species and differentiates them based on mannitol fermentation.
Baird-parker (BP) agar Which contains tellurite and egg yolk to detect coagulase-positive colonies.
Baird-parker agar supplemented with rabbit plasma fibrinogen (BP-RPF) Which enhances the detection of coagulase activity.
Commercial chromogenic media Are also widely used for rapid and specific identification.
Escherichia coli Eosin Methylene Blue (EMB) Are classic choices that differentiate E. coli based on lactose fermentation, with characteristic color changes.
MacConkey
β-Glucuronidase-based chromogenic media Target a specific enzyme commonly produced by E. coli, allowing for precise identification through color reaction.
Dry film systems such as Petrifilmâ„¢ EC Provide a convenient and standardized platform for the enumeration of E. coli and coliforms in food samples.