Table 1. List of different probiotic cheese and information of the name of probiotic, method and stage of probiotic incorporation in cheese, viability, and functional characteristics

Type of cheese Name of the probiotics Method and stage of probiotic supplementation Viability of probiotics (CFU/g/mL) Functional characteristics (a comparison with control cheese)
Spanish pasteurized sheep milk cheese Bifidobacterium pseudolongum INIA P2, Bifidobacterium longum INIA P678, and Bifidobacterium breve INIA P734 Added after pasteurization with cheese starter culture at 30°C Only B. pseudolongum INIA P2 remained stable (2.0×106) over a 60-day period of storage and during in vitro simulated digestion Significantly enhanced overall proteolysis and the concentration of some free amino acids.
Cream cheese Lactobacillus rhamnosus The probiotic strain was added after the curdling step (1011 CFU/g) 108 after 35 days of storage at 4°C -
Squacquerone cheese Lactobacillus crispatus Added before renneting (6.8 log CFU/mL) in combination with cheese starter culture 1×107 after 13 days of storage Better organoleptic characteristics of the tested cheese in terms of creaminess, flavor, and overall consumer acceptability and potential to prevent gynecological infection
Cheddar cheese Lactobacillus casei and Lactobacillus plantarum Added before renneting in combination with cheese starter culture - Increased antioxidant activity
Minas Frescal cheese L. casei 01 Inoculation of probiotics in pasteurized milk in combination with starter lactococci (1% v/v, 1×107 to 1×108 CFU/g) After the cheese preparation, the count was 1×108 Better fatty acid profile, higher ACE-inhibitory and antioxidant activities, lower pH, and higher proteolysis and organic acid levels
A pasta filata cheese L. rhamnosus GG The probiotic strain was added to the milk before renneting in combination with normal cheese starter (5×107 CFU/mL) 1×107 after 10 days of storage Increased production of diacetyl, acetoin, citric, succinic, and acetic acids
Edam cheese Bifidobacterium bifidum Inoculation of probiotics in pasteurized milk before renneting (107 CFU/g) in combination with normal cheese starter culture Survival of probiotics following 3 months of ripening Increase in free fatty acids
Fior di Latte cheese Lactobacillus delbrueckii subsp. bulgaricus, and Lactobacillus paracasei BGP1 Probiotics were added in curd in the last stage of curd ripening (1×108 to 1×109 CFU/g) High survival (1×108) at 4°C during 14 days of storage Enhanced flavor and shelf-life
Cheddar cheese Lactobacillus helveticus H100 and Lactobacillus acidophilus L10 Inoculation of probiotics in pasteurized milk in combination with starter lactococci (L10) >1×106 after 14 weeks of storage Higher contents of lactic, propionic, and acetic acids, water-soluble nitrogen, trichloroacetic acid, and phosphotungstic acid, improved proteolysis, and higher ACE-inhibitory activity
Cheddar cheese L. acidophilus 4962 and L10, Bifidobacterium longum 1941, L. casei 279, and Bifidobacterium lactis B94 Inoculation of probiotics in pasteurized milk in combination with starter lactococci (1×108 to 1×109 CFU/g) Survival of all probiotics after 6 months of storage at 4°C (1×107 to 1×108) More acetic acid concentration, free amino acids, and higher αs-casein
Minas Frescal cheese L. acidophilus (La5) Added alone (T1) and in combination with cheese starter culture (T2; 1×106 CFU/g) T1 (1×107) and T2 (1×106) after 14 days of storage Better sensory attributes of both T1 and T2
Cheddar cheese B. lactis Bb12 Inoculation of probiotics in pasteurized milk after renneting in combination with starter lactococci 108 after 12 weeks of storage Not reported
Kefalograviera-type cheese L. casei subsp. rhamnosus and Lactococcus lactis subsp. Cremoris The probiotic strains were added in milk in combination with normal starter culture Not determined More flavor intensity
Cheddar cheese B. lactis Bb-12 The probiotic strain was added in standardized milk without normal cheese starter culture (1×108 CFU/mL) Survival in high number (>108) following 6 months of storage Reduced moisture down to 40%, which is only slightly above the permitted moisture limit of Cheddar. However, flavor was improved compared to the control cheese.
Cheddar cheese B. longum BB536 The probiotic strain was added in standardized milk without normal cheese starter culture (1×108 CFU/mL) A reduction in number (1×105) following 1 month of ripening Better flavor
Argentinian Fresco cheese B. bifidum and L. acidophilus Probiotics were added before renneting (1% wt/wt or 107 CFU/g) >106 after 60 days of refrigerated storage Not reported
Argentinian Fresco cheese L. casei Probiotics were added before renneting (1% wt/wt or 107 CFU/g) Good viability and no decrease in count after 60 days of storage Not reported
Cheddar -like cheese Bifidobacterium infantis Probiotics were added in cream (1×107 CFU/g) that was used to standardize milk 3×106 after 84 days of storage High β-galactosidase activity and casein hydrolysis
Cheddar cheese L. paracasei Probiotics were added in milk before renneting (0.1% v/v: 2.7×107) Reached to 1.5×108 after 3 months of ripening Not reported
Cheddar cheese B. bifidum (ATCC 15696) After milling and salting (1×106 CFU/g) 1.7×l07 at the 18th week Not reported
Data from Gao et al. [56].