Table 2. Inclusivity and exclusivity as confirmation of E. sakazakii (Cronobacter spp.)

Strain tested in study Confirmation method
DFI1) Nutrient agar Rapid ID 32E Real-Time PCR
Pantoea agglomerase Yellow Yellow Non-E.sakazakii Negative
Salmonella Enteritidis 18088 Black Yellow Non-E.sakazakii Negative
Enteritidis 18673 Black Yellow Non-E.sakazakii Negative
Typhimurium LT2 Black Yellow Non-E.sakazakii Negative
Enterbacter gergorivae Yellow Yellow Non-E.sakazakii Negative
dissolvens E10 Yellow Yellow Non-E.sakazkaii Negative
helveticus E912 Green Yellow Non-E.sakazakii Negative
helveticus E440 Weak green Yellow Non-E.sakazakii Negative
vulneris Green Yellow Non-E.sakazakii Negative
sakazkaii N6 Green Yellow E. sakazkaii True positive
sakazkaii 626 Weak green Yellow E. sakazakii True positive
Druggan-Forsythe-Iversen (DFI, Oxoid) as chromogenic agar was first developed for the purpose of detecting E. sakazakii (Cronobacter spp.) which was shown green colony color on DFI.