Table 1. Condition of preparative liquid chromatography system
Instrument
Condition
Column
Hiprep 16/60 Sephacryl S-100 HR column (GE Healthcare Life Sciences, USA)
Mobile phase
5 mM sodium phosphate buffer (0.15 M NaCl, pH 7.0)
Detector (detection)
Waters dual
λ
absorbance detector W2487 (280 nm)
Flow rate
1 mL / min
Injection volume
3 mL