Table 1. Condition of preparative liquid chromatography system

Instrument Condition
Column Hiprep 16/60 Sephacryl S-100 HR column (GE Healthcare Life Sciences, USA)
Mobile phase 5 mM sodium phosphate buffer (0.15 M NaCl, pH 7.0)
Detector (detection) Waters dual λ absorbance detector W2487 (280 nm)
Flow rate 1 mL / min
Injection volume 3 mL