Instrument | Condition |
---|---|
Column | Hiprep 16/60 Sephacryl S-100 HR column (GE Healthcare Life Sciences, USA) |
Mobile phase | 5 mM sodium phosphate buffer (0.15 M NaCl, pH 7.0) |
Detector (detection) | Waters dual |
Flow rate | 1 mL / min |
Injection volume | 3 mL |