Table 1. Bacteria strains, plasmids and DNA oligomers used in this study

Strains Genotype Source or references
FB248 Salmonella enterica serovar Gallinarum(S. Gallinarum) WT Cho et al., 2015
FB254 Salmonella enterica serovar Enteritidis(S. Enteritidis) WT Sun and Hahn, 2012
IB2126 S. Gallinarum /pspy-gfp1 This study
IB2129 S. Enteritidis /pspy-gfp1 This study
IB2203 S. Gallinarum ΔcpxR :: CM /pspy-gfp1 This study
IB2204 S. Enteritidis ΔcpxR :: CM /pspy-gfp1 This study
IB2205 S. Gallinarum ΔbaeR :: CM /pspy-gfp1 This study
IB2206 S. Enteritidis ΔbaeR :: CM /pspy-gfp1 This study
Plasmids Characteristics Source or References
pFPV25 gfp fusion vector Gift from Raphael Valdivia (Valdivia and Falkow, 1996)
Pspy-gfp 1 gfp fusion containing the entire spy gene Jeong et al., 2017
Pspy-gfp 2 gfp fusion containing the spy promoter This study
DNA oligomers Sequences (5’–3’)
spy linker SacI Fw-1 TATGAGCTCTGCTATATCATGCTGTTGTA
spy linker BamHI Rev-1 TATGGATCCAGGACGGCTATAGAATTCTCTG
spy linker SacI Fw-2 TATGAGCTCTGCTATATCATGCTGTTGTA
spy linker BamHI Rev-2 TATGGATCCTACCTTTATGCTG CATCATT