Table 2 The instrumental conditions for analyzing lactase activity by HPLC

Items	Conditions
Instrument	Agilent 1200 HPLC ChemStationTM (Agilent, USA)
Column	Guard column (4.6 mm ID×12.5 mm, Agilent, USA)Hi-Plex Ca Carbohydrate Analysis column (6.5 mm× 300 mm)
Data program	ChemStationTM Data Browser Software Module (Agilent, USA)
Detector	Refractive Index Detector (RID, Agilent, USA)
Mobile phase	0.013 N H2SO4
Flow rate	0.8 mL/min
Column temp.	65℃
Injection volume	20 μL