Table 1 HPLC operation conditions for analysis of sialic acid

1. HPLC system(Agilent 1260)	2. Analysis conditions
∙Pump: G1311C ∙Auto sampler: G1329B∙Column: G1316A∙UV detector: G1314F	∙Wavelength: 205 nm ∙Column: Aminex® HPX-87H Ion Exclusion Column(300×7.8 mm, 9 μm, Bio-Rad Co.) ∙Mobile phase: 10 mM H2SO4∙Running time: 20 min∙Flow rate: 0.5 mL/min ∙Injection volume: 10 μL ∙Temperature: 40℃∙Standard sol.(w/w): Silaic acid 0.1 ppm, 1 ppm, 10 ppm