Table 5 The result of evaluation (main study) conducted to evaluate the effect on increase & decrease of reverse mutation colonies by the addition of 7%-GNANA, a test substance, under the presence and absence conditions of metabolic activation system

With and without metabolic activation		Test material conc.(μg/plate)	Number of revertant colony (cfu/plate)
			Base substitution types			Frameshiftm types
			TA100*	TA1535*	WP2uvrA*	TA98*	TA1537*
S9 Mix(－)		0	124.3± 2.31#	25.7±2.31#	24.7±1.53#	32.0±1.73#	13.7±2.08#
		61.7	111.3± 7.77	25.7±2.08	26.0±5.00	34.0±2.65	13.0±1.00
		185	115.7±12.66	25.0±2.00	27.3±2.08	35.3±1.53	13.7±0.58
		556	132.0±13.00	27.7±2.08	26.7±3.21	39.0±1.00	14.7±2.89
		1,670	119.3± 7.51	26.0±5.00	26.7±3.79	36.7±0.58	13.3±0.58
		5,000	128.7±10.97	28.3±2.89	24.0±1.00	37.3±2.08	13.3±0.58
S9 Mix(+)		0	134.7± 0.58	24.0±1.73	25.3±1.53	35.7±1.53	14.7±2.52
		61.7	120.0± 2.65	23.3±3.21	25.0±6.93	38.3±2.08	13.7±1.53
		185	117.7± 1.15	24.0±3.61	25.7±3.79	37.0±2.65	12.7±3.06
		556	109.7± 8.96	22.0±1.00	28.0±4.00	37.0±3.61	13.0±1.00
		1,670	125.7± 7.77	23.3±4.93	29.3±1.53	33.7±4.73	13.0±1.00
		5,000	125.7±13.20	27.0±2.65	29.7±4.16	36.7±2.52	13.7±0.58
Positive control	S9 Mix(－)	Materialds	NaN3	NaN3	4-NQO	4-NQO	9-AA
		Conc. (μg/plate)	1.5	1.5	0.5	0.5	80
		cfu/plate	442.3±10.26	237.0±13.53	241.3±25.01	287.3±15.14	272.7±9.87
	S9 Mix(+)	Materialds	2-AA	2-AA	2-AA	2-AA	2-AA
		Conc. (μg/plate)	1.0	2.0	10	0.5	2.0
		cfu/plate	406.7±3.51	241.0±14.11	344.7±9.50	265.7±17.62	290.0±23.43

^*: TA98 (Sal. typhimurium TA98), TA100 (Sal. typhimurium TA100), TA1535 (Sal. typhimurium TA1535), TA1537 (Sal. typhimurium TA1537), WP2uvrA (E. coli WP2uvrA), ^#: Represents a significant difference at p<0.01 level compared with the vehicle control